Architecture and evolution of blade assembly in b-propeller lectins

Session: 
S8.2 Lectins II
Code: 
OL8.2.1
Location (hall): 
Mannose
Start/end time: 
Thursday, July 4, 2019 - 11:45 to 12:00
Anne
Imberty

François Bonnardel1,2, Atul Kumar3, Michaela Wimmerova3, Martina Lahmann4, Serge Pérez1, Annabelle Varrot1, Frédérique Lisacek2Anne Imberty1

1Cermav-cnrs, Grenoble, France, 2Swiss Institute of Bioinformatics, Geneva, Switzerland, 3CEITEC, Masaryk University, Brno, Czech Republic, 4University of Bangor, Bangor, United Kingdom

Lectins with a β-propeller fold bind glycans on the cell surface through multivalent binding sites and appropriate directionality. These proteins are formed by repeats of short domains, raising questions about evolutionary duplication. However, these repeats are difficult to detect in translated genomes and seldom correctly annotated in sequence databases. To address these issues, a database has been developped and is now available on internet at https://www.unilectin.eu/propeller.

We defined the blade signature of the five types of β-propellers using 3D-structural data. With these templates, we predicted 3887 β-propeller lectins in 1889 species and organised this new information in a searchable online database. The data reveals a widespread distribution of β-propeller lectins across species. Prediction also emphasises multiple architectures and led to uncover a novel β-propeller assembly scenario. This was confirmed by producing and characterizing a predicted protein coded in the genome of Kordia zhangzhouensis. The crystal structure shows a new intermediate in the evolution of β-propeller assembly and demonstrates the power of our tools.

Discovery of new b-propeller lectin assembly from data base prediction

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