Practical Synthesis of Complex Glycopolymers Using Water-Soluble Amino-Oxy Functional Scaffolds

<none>

Programme Social programme Plenary speakers Keynote speakers 1st EuroCarb Young Researcher (ECYR) Workshop CarboMet Workshop Mini symposium: Glycobiology at the LUMC and beyond Favourites/Agenda

Social programme

Plenary speakers

Keynote speakers

1st EuroCarb Young Researcher (ECYR) Workshop

CarboMet Workshop

Mini symposium: Glycobiology at the LUMC and beyond

Favourites/Agenda

Photos

Venue & Travel Venue Accommodation City of Leiden Travel directions

Venue

Accommodation

City of Leiden

Travel directions

Committees

Home

Practical Synthesis of Complex Glycopolymers Using Water-Soluble Amino-Oxy Functional Scaffolds

Antonio Laezza 1 , Sarah Jane Richards 1 , Matthew Gibson 1,2

1 University Of Warwick, Chemistry Department, Coventry, United Kingdom, 2 University Of Warwick, Warwick Medical School, Coventry, United Kingdom

Glycopolymers are an exciting class of synthetic macromolecules which can display increased affinity towards carbohydrate binding proteins (especially lectins). This is due to the cluster glycoside effect, which results in a non-linear increase in affinity as the valency increases. These multivalent glycan interactions are involved in a range of biological processes such as cell-cell and cell-pathogens communications, and hence their application as decoys in anti-adhesive therapy or to modulate signalling is of huge interest.

Most previous studies on glycopolymers involve relatively simple monosaccharides, which do not enable reproductions of the complex 3-D presentation found in native glycans and hence do not have high selectivity. Furthermore most binding studies are against model plant lectins, and use simple aggregation assays.

To address the above, we will present a practical method to obtain water soluble amino-oxy functional polymers which can be directly reacted with reducing glycans enabling aqueous post-polymerization modification and assembly of the glycans into polymers or nanoparticles. This strategy is useful as it allows the use of both larger glycans and those bearing sulphated and other residues which are crucial for affinity in some cases. The route involves a two-step modification of poly (N-hydroxyethyl acrylamide) (PHEA) obtained from RAFT polymerization then subsequent glycan capture. We will present both the synthesis and the utility of this to study their interactions to human and pathogenic carbohydrate binding proteins using colourmetric assays and biolayer interferometry.

Contact eurocarb@eurocarb2019.com for general questions about EUROCARB2019, or codee@eurocarb2019.com for scientific questions.

Leiden University / Leiden Institute of Chemistry lic.leidenuniv.nl

European Carbohydrate Organisation

Congress by Design www.congressbydesign.com