New avenues to characterize the conformation and recognition features of multi-antennary and extended N-glycans by using NMR

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Mini symposium: Glycobiology at the LUMC and beyond

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New avenues to characterize the conformation and recognition features of multi-antennary and extended N-glycans by using NMR

Angeles Canales 1 , Beatriz Fernández de Toro 2 , Wenjie Peng6, Andrew Thompson 4 , Theodoros Karagiannis 5 , Javier Cañada 2 , Carlo Unverzagt 5 , James Paulson 4 , Jesús Jiménez-Barbero 3

1 Complutense University of Madrid, Madrid, Spain, 2 Centro de Investigaciones Biológicas, CSIC, Madrid, Spain, 3 CIC bioGune, Bilbao, Spain, 4 The Scripps Research Institute , La Jolla, EEUU, 5 Lehrstuhl fur Bioorganische Chemie, Universitat Bayreuth, Bayreuth, Germany, 6 Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University , Shangai, China

Complex glycosylation patterns containing multiantennary N-glycans are typically found in mature glycoproteins. However, the structural characterization of these glycans is rather challenging. Usually, standard NMR and X-ray diffraction techniques fail to provide specific answers on the structure and molecular recognition features due to intrinsic attributes of the glycan. As a promising approach, carbohydrates conjugated to lanthanide binding tags have revealed high potential toward this aim. This methodology has first been applied to the study of small oligosaccharides (di-, tri- and tetrasaccharides). [1-3] Proceeding from this experimental basis, we have extended this concept to the level of high degree branching and long chain N-glycans. The unprecedented resolution obtained in the spectra has allowed us to perform conformational and interaction analysis of complex carbohydrates such as: tetraantennary N-glycans and a sialylated tetradecasaccharide N-glycan presenting two LacNAc repetitions at each arm. [4-5] The latter is especially relevant since it has been identified as the receptor of the hemagglutinin protein of pathogenic influenza viruses. [5]

Sialylated tetradecasaccharide N-glycan derivative

S. Yamamoto et al. Chemistry Eur. J. 2011, 17, 9368-76.

Y. Zhang et al. Molecules, 2012, 17, 6658-71.

A. Canales et al. J. Am. Chem. Soc. 2014, 136, 8011-7.

A. Canales et al. Angew. Chem. Int. Ed. Engl. 2017, 56(47), 14987-14991.

B. Fernández de Toro et al. Angew. Chem. Int. Ed. Engl. 2018, 57(46), 15051-15055.

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