To date, glycosyltransferases (GTs) are an essential family of enzymes poorly characterize both structurally and mechanistically which is being a major bottleneck in Glycoscience. They play a crucial role in living organisms catalysing the stereo- and regiospecific transfer of an activate donor sugar to an acceptor moiety to build up complex oligosaccharides onto the cell surface . It requires the development of new methodologies able to elucidate and characterize the interactions between the glycosyl moiety and the enzyme. Surface plasmon resonance imaging (SPRi) has spread as a powerful analytical tool for deciphering many biomolecular processes exploring the kinetics and monitoring multiple interactions of biological and chemical substrates at real time and without any labels . We aim at the development of a biosensor enable to rationalize the characterization of glycosyltransferases activities involved in the plant cell wall biosynthesis. To this aim, a fucosyltransferase from the plant Arabidosis thaliana (AtFUT1) (CAZY family GT37), was selected as model glycosyltransferase. This enzyme participates in the last step in the biosynthesis of xyloglucan (XG) transferring L-Fucose molecule from GDP-Fucose (donor) to a galactose sugar (acceptor) . To that end, multiple chemical strategies to functionalize large moieties of xyloglucan to graft it on the gold surface have been developed  . Depicting and monitoring the interactions between the AtFUT1 and the xyloglucan in terms of both specificity and activity represent a strong challenge. Complementary techniques such as isothermal titration calorimetry (iTC) may guide to streamline the comprehension and support the rationalization of the glycosylation process .
- Albesa-Jové, D; Guerin, M. Current Opinion in Structural Biology. 2016, 40, 23-32.
- Bosch, M; Sánchez, A.J; Rojas, F; Ojeda, C. Sensors. 2007, 7, 797-859.
- Cicéron, F; Rocha, J; Kousar, S; Hansen, S; Chazalet, V; Gillon, E; Breton, C; Lerouxel, O. Biochimie. 2016, 128-129, 183-192.
- Mallevre, F; Roget, A; Minon, T; Kervella, Y; Ropartz, D; Ralet, M.C; Canut, H; Livache, T. Bioconjugate Chem. 2013, 24, 1264-1269.
- Thakar, D; Migliorini, E; Coche-Guerente, L; Sadir, R; Lortat-Jacob, H; Boturyn, D; Renaudet, O; Labbe, P and Richter, R. Chem Commun. 2014, 50, 15148.
- Mazzei, L; Ciurli, S; Zambelli, B. Journal of Visualized Experiments. 2014, 86, 1-8.