New NMR Applications to the Study of Carbohydrate-Protein Interactions

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Mini symposium: Glycobiology at the LUMC and beyond

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New NMR Applications to the Study of Carbohydrate-Protein Interactions

Mónica Martínez-Orts 1 , Theodoros Karagiannis 2 , Gemma Domínguez 3 , Javier Pérez-Castells 3 , Javier Cañada 4 , Carlo Unverzagt 2 , Jesús Jiménez-Barbero 5,6 , Angeles Canales 1,4

1 Dep. Química orgánica, Fac. Ciencias Químicas, Universidad Complutense de Madrid, Madrid, Spain, 2 Bioorganische Chemie, Gebäude NW1 Universität Bayreuth, Bayreuth, Germany, 3 Dep. Química, Fac. Farmacia, Univ. CEU-San Pablo, Madrid, Spain, 4 Dep. Biología Físico Química, CIB-CSIC, Madrid, Spain, 5 CIC bioGUNE, Bizkaia Science and Technology Park , Derio, Spain, 6 IKERBASQUE, Basque Foundation for Science, Bilbao, Spain

Complex-type N-glycans are one of the most relevant carbohydrates involved in key biological processes such as cell growth, migration or tumor invasion [1]. In fact, aberrant glycosylation is characteristic of cancer cells or cancer tissues which play an important role in the disease progression and its metastasis [2]. Despite of their biological importance, the molecular recognition of these biomolecules is still a challenge. The flexibility of N-glycans and their structural similarities makes difficult its structural characterization both by NMR and X-ray methodologies. Herein we describe a new NMR approach to study these complex structures by using carbohydrate conjugates bearing lanthanide binding tags. In this context, we have already studied a tetraantennary N-glycan using this methodology [3]. In this work, a family of N-glycans will be characterized (see Figure 1). Thanks to the paramagnetic effect we could define both the conformation and the recognition features of these complex carbohydrates.

Figure 1. Carbohydrate conjugates characterized in this work

Zhao, Y. Y.; Sato, Y.; Isaji, T.; Fukuda, T.; Matsumoto, A.; Miyoshi, E.; Gu, J.; Taniguchi, N. FEBS J, 2008, 275, 1939−1948

Taniguchi N., Kizuka Y., Systems Glycobiology Research Group, Adv. in Cancer Research, 2015, 126, 12-39

Angeles Canales, Alvaro Mallagaray, Javier Pérez-Castells, Irene Boos, Carlo Unverzagt, Sadine André, Hans-Joachim Gabius, Francisco Javier Cañada, and Jesús Jiménez-Barbero, Angew. Chem. Int. Ed. 2013, 52, 13789-13793

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