Complex-type N-glycans are one of the most relevant carbohydrates involved in key biological processes such as cell growth, migration or tumor invasion . In fact, aberrant glycosylation is characteristic of cancer cells or cancer tissues which play an important role in the disease progression and its metastasis . Despite of their biological importance, the molecular recognition of these biomolecules is still a challenge. The flexibility of N-glycans and their structural similarities makes difficult its structural characterization both by NMR and X-ray methodologies. Herein we describe a new NMR approach to study these complex structures by using carbohydrate conjugates bearing lanthanide binding tags. In this context, we have already studied a tetraantennary N-glycan using this methodology . In this work, a family of N-glycans will be characterized (see Figure 1). Thanks to the paramagnetic effect we could define both the conformation and the recognition features of these complex carbohydrates.
- Zhao, Y. Y.; Sato, Y.; Isaji, T.; Fukuda, T.; Matsumoto, A.; Miyoshi, E.; Gu, J.; Taniguchi, N. FEBS J, 2008, 275, 1939−1948
- Taniguchi N., Kizuka Y., Systems Glycobiology Research Group, Adv. in Cancer Research, 2015, 126, 12-39
- Angeles Canales, Alvaro Mallagaray, Javier Pérez-Castells, Irene Boos, Carlo Unverzagt, Sadine André, Hans-Joachim Gabius, Francisco Javier Cañada, and Jesús Jiménez-Barbero, Angew. Chem. Int. Ed. 2013, 52, 13789-13793