Chemical Composition and In Vitro Anticoagulant Activity of Exopolysaccharides from Spirulina Platensis

<none>

Programme Social programme Plenary speakers Keynote speakers 1st EuroCarb Young Researcher (ECYR) Workshop CarboMet Workshop Mini symposium: Glycobiology at the LUMC and beyond Favourites/Agenda

Social programme

Plenary speakers

Keynote speakers

1st EuroCarb Young Researcher (ECYR) Workshop

CarboMet Workshop

Mini symposium: Glycobiology at the LUMC and beyond

Favourites/Agenda

Photos

Venue & Travel Venue Accommodation City of Leiden Travel directions

Venue

Accommodation

City of Leiden

Travel directions

Committees

Home

Chemical Composition and In Vitro Anticoagulant Activity of Exopolysaccharides from Spirulina Platensis

Zakaria Boual 1,3 , Karima Guehaz 1 , Toufik Chouana 1 , Ali Seggai 1 , Ahlem Boudoumi 1 , Hania Lati 1 , Noura Addoun 1 , Fatima benaoun 1 , Michaud Philippe 2 , Mohamed Didi Oul El Hadj 1

1 kasdi merbah University, Ouargla, Algeria, 2 Université Clermont Auvergne, CNRS, SIGMA Clermont, Institut Pascal, F-63000 Clermont-Ferrand, France, Clermont-Ferrand, france, 3 Laboratoire des analyses médicales IBN ROCHD, Rue Khemisti, 47000 Ghardaïa, Algeria., Ghardaia, Algeria

Exopolysaccharides (EPS) extracted with hot water from Spirulina platensis were characterized. The mass performance of water-soluble exopolysaccharides of Spirulina platensis suspension is 13.95%. The chemical composition of ESP was mainly consisted of 72.23±1.37% carbohydrates, among them 26.6±1.48% are neutral monosaccharides and 45.47±0.98% are uronic acids. Chromatographic analyzes using four TLC systems after 2M TFA hydrolysis at 80°C for 4 h indicate that ESP are composed mainly of glucoronic acid, arabinose and mannose. In vitro study shows that EPS of S. platensis exhibited anticoagulant activity as compared with heparin, a standard anticoagulant. The anticoagulant activity was detected by prolongation of activated partial thromboplastin time (APTT) and prothrombin time (PT). The EPS extract at 0.1% showed >300 s of APTT activity. In PT assay, almost all tested concentration did not show good activities. According to the results, the EPS extract strongly inhibit the endogenous pathway of coagulation.

Contact eurocarb@eurocarb2019.com for general questions about EUROCARB2019, or codee@eurocarb2019.com for scientific questions.

Leiden University / Leiden Institute of Chemistry lic.leidenuniv.nl

European Carbohydrate Organisation

Congress by Design www.congressbydesign.com