Chemical Composition and In Vitro Anticoagulant Activity of Exopolysaccharides from Spirulina Platensis

PS2 Poster session 2 Even numbers
Location (hall): 
Start/end time: 
Tuesday, July 2, 2019 - 15:45 to 17:15

Zakaria Boual1,3, Karima Guehaz1, Toufik Chouana1, Ali Seggai1, Ahlem Boudoumi1, Hania Lati1, Noura Addoun1, Fatima benaoun1, Michaud Philippe2, Mohamed Didi Oul El Hadj1

1kasdi merbah University, Ouargla, Algeria, 2Université Clermont Auvergne, CNRS, SIGMA Clermont, Institut Pascal, F-63000 Clermont-Ferrand, France, Clermont-Ferrand, france, 3Laboratoire des analyses médicales IBN ROCHD, Rue Khemisti, 47000 Ghardaïa, Algeria., Ghardaia, Algeria

Exopolysaccharides (EPS) extracted with hot water from Spirulina platensis were characterized. The mass performance of water-soluble exopolysaccharides of Spirulina platensis suspension is 13.95%. The chemical composition of ESP was mainly consisted of 72.23±1.37% carbohydrates, among them 26.6±1.48% are neutral monosaccharides and 45.47±0.98% are uronic acids. Chromatographic analyzes using four TLC systems after 2M TFA hydrolysis at 80°C for 4 h indicate that ESP are composed mainly of glucoronic acid, arabinose and mannose. In vitro study shows that EPS of S. platensis exhibited anticoagulant activity as compared with heparin, a standard anticoagulant. The anticoagulant activity was detected by prolongation of activated partial thromboplastin time (APTT) and prothrombin time (PT). The EPS extract at 0.1% showed >300 s of APTT activity. In PT assay, almost all tested concentration did not show good activities. According to the results, the EPS extract strongly inhibit the endogenous pathway of coagulation.