18B7 a monoclonal antibody against the glucuronoxylomannan (GXM), a major component of the polysaccharide capsule that surrounds Cryptococcus neoformans, has been identified to have catalytic activity.1 18B7 is able to hydrolyse GXM oligosaccharides, providing the first example of a naturally occurring catalytic antibody for polysaccharides. To better understand the kinetic activity, synthesis of oligosaccharide Förster Resonance Energy Transfer (FRET) probes has been undertaken. Details of the synthesis and the problems encountered towards these oligosaccharide FRET will be presented.
Bowen, A. et al. Journal of Biological Chemistry. 2016, 292, 417-434.