Determination of Lactose in Low-Lactose Dairy Products

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Programme Social programme Plenary speakers Keynote speakers 1st EuroCarb Young Researcher (ECYR) Workshop CarboMet Workshop Mini symposium: Glycobiology at the LUMC and beyond Favourites/Agenda

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1st EuroCarb Young Researcher (ECYR) Workshop

CarboMet Workshop

Mini symposium: Glycobiology at the LUMC and beyond

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Determination of Lactose in Low-Lactose Dairy Products

Elwin van der Cruijsen 1 , Judith Vis 1 , Ekaterina Churakova 1 , Remko Winter 1 , Wibo B. van Scheppingen 1 , Marieke P. Vijverberg 1 , Leon Coulier 1 , Alexander L.L. Duchateau 1 , Maurien Olsthoorn 1 , Adriana Carvalho De Souza 1

1 DSM Biotechnology Center, Delft, The Netherlands

The demand for low lactose dairy products is increasing and more different lactose free food is commercially available. The level of lactose in these products decreased during the last years and nowadays a concentration of < 0.01% is generally accepted as “lactose free”. DSM has a portfolio of lactase to produce lactose-free dairy products. Measurement of lactose enzymatic conversion into the diverse products in real application and in time resolved time is key for the development and optimization of lactases.

In this presentation, we will show the application and performance evaluation of diverse lactose measurements techniques in real dairy products. We applied from Chromatographic methods (including ISO methods) to NMR and enzymatic-assays based determination. In this study we conclude that depending on the application and the information needed different techniques should be applied. Finally, to be able to study in detail lactase activity in real application a diverse analytical toolbox and strategy is needed.

J. Chromatogr. B, 1060 (2017) 395-399

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European Carbohydrate Organisation

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