Resolving Extended N-Glycans by NMR: New Insights into Influenza Hemagglutinin N-Glycan Interactions

<none>

Programme Social programme Plenary speakers Keynote speakers 1st EuroCarb Young Researcher (ECYR) Workshop CarboMet Workshop Mini symposium: Glycobiology at the LUMC and beyond Favourites/Agenda

Social programme

Plenary speakers

Keynote speakers

1st EuroCarb Young Researcher (ECYR) Workshop

CarboMet Workshop

Mini symposium: Glycobiology at the LUMC and beyond

Favourites/Agenda

Photos

Venue & Travel Venue Accommodation City of Leiden Travel directions

Venue

Accommodation

City of Leiden

Travel directions

Committees

Home

Resolving Extended N-Glycans by NMR: New Insights into Influenza Hemagglutinin N-Glycan Interactions

Beatriz Fernández de Toro Ronda 1 , Wenjie Peng 2,3 , Andrew Thompson 3 , Gema Domínguez 4 , F. Javier Cañada 1,5 , Javier Pérez Castells 4 , James C. Paulson 3 , Jesús Jiménez Barbero 6,7 , Ángeles Canales 8

1 Dpto Biología Estructural y Química, Centro de Investigaciones Biológicas CSIC, Madrid, Spain, 2 Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University, Shanghai, China, 3 Depts. of Mol Med and Immunol and Microbiol, The Scripps Research Institute, San Diego, USA, 4 Dpto Química, Fac. Farmacia, Universidad San Pablo CEU, Madrid, Spain, 5 CIBER of Respiratory Diseases (CIBERES), , Spain, 6 CIC bioGUNE, Bizkaia Science and Technology Park, Bilbao, Spain, 7 IKERBASQUE, Basque Foundation for Science, Bilbao, Spain, 8 Dpto Química Orgánica I, Fac. Ciencias Químicas, Universidad Complutense de Madrid, Madrid, Spain

Seasonal flu (influenza virus infection) is one of the major threats to human health, causing of up to 500 thousand deaths worldwide annually).[1,2] Hemagglutinin, a glycoprotein presented in the surface of the influenza virus, binds sialylated N-glycans located on the surface of host epithelial cells.

The structural elucidation and the conformational studies of N-glycans remain a challenge due to the complexity, heterogeneity and flexibility of carbohydrates. Moreover, the isochronous chemical shifts presented for the same units located at different branches make impossible the distinction of their signals in the NMR spectra.

Taking advantage of the lanthanide approach, an unambiguous assignment of each carbohydrate unit has been reached for bianntenary N-glycans containing one and two LacNAc units, permitting their conformational elucidation and the recognition study of these N-glycans by the HK/68 hemagglutinin strain.[3]

Left: Structure of the N-glycan containing 2 LacNAc units. Right: Extension of the H4 galactoses region of the ¹H-¹³C HSQC spectrum.

Influenza (Flu) | CDC https://www.cdc.gov/flu/ (accessed May 8, 2019).

Petrova, V. N.; Russell, C. A. Nat. Rev. Microbiol. 2017, 16 (1), 47–60.

Fernández de Toro, B.; Peng, W.; Thompson, A. J.; Domínguez, G.; Cañada, F. J.; Pérez-Castells, J.; Paulson, J. C.; Jiménez-Barbero, J.; Canales, Á. Angew. Chemie Int. Ed. 2018, 57 (46), 15051–15055.

Contact eurocarb@eurocarb2019.com for general questions about EUROCARB2019, or codee@eurocarb2019.com for scientific questions.

Leiden University / Leiden Institute of Chemistry lic.leidenuniv.nl

European Carbohydrate Organisation

Congress by Design www.congressbydesign.com