Molecular recognition by specific targets is at the heart of the life processes. Interactions between proteins and carbohydrates mediate a broad range of biological activities, from embryogenesis to pathological processes. The elucidation of the mechanisms that govern how sugars are accommodated in the binding sites of these receptors is currently a topic of interest. Thus, the determination of structural and conformational factors and physicochemical features which govern the molecular recognition process is of paramount importance.¹ We aim at mimicking the cellular environment using different probes², by performing in vitro experiments,³ and using NMR methods in solution⁴ and in cell, and also under experimental conditions that mimic the cell environment, developing synthetic membranes and exploiting highly crowded atmospheres.⁵ We herein present our preliminary results of the structural details of the interactions between human galectin-3¹ ⁶ with GM3-containing model membranes using NMR spectroscopy.
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