Model Membranes to Mimic Cellular Environments in Carbohydrate Recognition Processes

PS1 Poster session 1 Odd numbers
Location (hall): 
Start/end time: 
Monday, July 1, 2019 - 15:45 to 17:15

Marta G. Lete1, Ana Ardá1, Jesús Jiménez-Barbero1,2

1CICbioGUNE, Derio, Spain, 2Basque Foundation for Science (IKERBASQUE), Bilbao, Spain

Molecular recognition by specific targets is at the heart of the life processes. Interactions between proteins and carbohydrates mediate a broad range of biological activities, from embryogenesis to pathological processes. The elucidation of the mechanisms that govern how sugars are accommodated in the binding sites of these receptors is currently a topic of interest. Thus, the determination of structural and conformational factors and physicochemical features which govern the molecular recognition process is of paramount importance.¹ We aim at mimicking the cellular environment using different probes², by performing in vitro experiments,³ and using NMR methods in solution⁴ and in cell, and also under experimental conditions that mimic the cell environment, developing synthetic membranes and exploiting highly crowded atmospheres.⁵ We herein present our preliminary results of the structural details of the interactions between human galectin-3¹ ⁶ with GM3-containing model membranes using NMR spectroscopy.

  1. Gimeno, A. et al. Angew. Chem. Int. Ed. 2019, doi: 10.1002/anie.201900723. 
  2. Rahkila J. et al. ChemBioChem. 2019, 20, 203. 
  3. Gimeno, A. et al. ACS Chem Biol. 2017, 12, 1104. 
  4. Arda, A and Jimenez-Barbero J. Chem. Commun 2018, 54, 5761.
  5. Diniz, A. et al. Chem. Eur. J. 2017, 23, 13213.
  6. Santarsia S. et al. ChemMedChem. 2018, 13, 2030.