N-Glycan Characterization of Cow Milk Immunoglobulins by CE-LIF

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Programme Social programme Plenary speakers Keynote speakers 1st EuroCarb Young Researcher (ECYR) Workshop CarboMet Workshop Mini symposium: Glycobiology at the LUMC and beyond Favourites/Agenda

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1st EuroCarb Young Researcher (ECYR) Workshop

CarboMet Workshop

Mini symposium: Glycobiology at the LUMC and beyond

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N-Glycan Characterization of Cow Milk Immunoglobulins by CE-LIF

Apolka Domokos 1 , Hajnalka Jankovics 2 , Ferenc Vonderviszt 2 , András Guttman 1,3

1 Horváth Csaba Laboratory of Bioseparation Sciences, Research Centre for Molecular Medicine, Faculty of Medicine, University of Debrecen, Debrecen, Hungary, 2 Bio-Nanosystems Laboratory, Research Institute of Biomolecular and Chemical Engineering, University of Pannonia, Veszprém, Hungary, 3 Sciex, Brea, USA

Alternative glycosylation of different glycoproteins and their role in various diseases attracts the attention of scientists and recent publications are reporting continuously about new findings in this area [1]. Bovine and human milk derived oral immunoglobulin preparations were used to treat gastrointestinal infections until the beginning of 2000s [2]. Furthermore, diet derived IgG could affect human innate immune system responses in a long term manner [3]. Albeit, N-glycan research in a well-studied field including immunoglobulins derived from cow milk and other animal sources, but still have some questions to be clarified [4]. In this study we have analyzed the IgG and IgA molecules captured from organic and non-organic cow milk colostrum (from local farms in Hungary) using protein G and IgA specific Z-domain variant Ni-IMAC columns. The glycan structures were released by PNGaseF digestion and labeled with a charged fluorophore aminopyrene trisulfonate (APTS), followed by purification with magnetic beads [5]. Analysis of the labeled glycans were carried out in an automated capillary electrophoresis system. Alterations in the N-glycan structures were found between organic and non-organic cow colostrum immunoglobulin samples, which could be the consequence of the different antibiotic or other treatments of the animals. Detailed structural characterization of the N-glycans found in both sample types will be given in a comprehensive comparable manner.

Zs. Kovacs, A. Simon, Z. Szabo, Z. Nagy, L. Varoczy, I. Pal, E. Csanky, A. Guttman, Electrophoresis, 2017, 38, 2115.

V. S. Jasion, B. P. Burnett, Nutrition Journal, 2015, 7, 14.

M. Splunter, T. L. J Osh, S. Brugman, H. F. J. Savelkoul, L- A. B. Joosten, M. G. Netea, R. J. J. Neerven, Nutrients, 2018, 10, 1378.

T. D-Dopico, M. R. Clatworthy, Frontiers in Immunology, 2019, 10, 805.

M. Szigeti, C. Lew, K. Roby, A. Guttman, Journal of Laboratory Automation, 2016, 21, 281.

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